Journal: Scientific Reports

Article Title: 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake

doi: 10.1038/s41598-022-19293-3

Figure Lengend Snippet: Optimizing OPTEC expansion. ( a ) Brightfield images showing OPTECs grown on plastic, matrigel, and laminin-511 substrates. ( b ) Heat map showing transporter expression of OPTECs between passages 0 (after isolation) and 5. ( c ) Brightfield images showing OPTEC populations at passages 0–5, scale bar = 20 μm. ( d ) Schematic view of the extracellular matrix (ECM), media, and media supplement conditions explored for optimizing OPTEC culture conditions. ( e ) Heat map comparing transporter expression as a function of the experimental conditions tested in ( d ).

Article Snippet: Figure 2 Optimizing OPTEC expansion. ( a ) Brightfield images showing OPTECs grown on plastic, matrigel, and laminin-511 substrates. ( b ) Heat map showing transporter expression of OPTECs between passages 0 (after isolation) and 5. ( c ) Brightfield images showing OPTEC populations at passages 0–5, scale bar = 20 μm. ( d ) Schematic view of the extracellular matrix (ECM), media, and media supplement conditions explored for optimizing OPTEC culture conditions. ( e ) Heat map comparing transporter expression as a function of the experimental conditions tested in ( d ).

Techniques: Expressing, Isolation

Journal: Scientific Reports

Article Title: 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake

doi: 10.1038/s41598-022-19293-3

Figure Lengend Snippet: 3D OPTEC-on-chip model. ( a ) Schematic views showing the processing steps used to create multiplexed, 3D OPTEC-on-chip models. ( b ) Corresponding images (left to right) of a representative chip after placing the channel templates, infilling the chip with ECM, removing the templates to create two-colocalized channels, and seeding one channel with OPTECs to create a 3D proximal tubule. ( c ) Confocal image of 3D OPTEC tubule showing actin (red) and DAPI (blue), scale bar = 50 μm. ( d ) Cross-sectional image of 3D OPTEC tubule (day 14, perfusion) highlighting the formation of a confluent monolayer, scale bar = 50 μm. ( e ) Brightfield image of 3D OPTEC tubule upon reaching confluency (day 7), scale bar = 75 μm. ( f ) OPTEC tubule (day 14, perfusion) stained for Na + /K + ATPase (green), LTL (magenta), and DAPI (blue). ( g ) OPTEC tubules exhibit proper apical polarization of primary cilia marker, acetylated alpha tubulin (red). ( h , i ) Basement membrane proteins laminin (red) and Col IV (green) are deposited by OPTECs on chip. ( j ) Proper expression of AQP1 (yellow) is observed in OPTEC tubules. ( f , j ) scale bars = 20 μm. ( k ) SEM image highlighting primary cilia on OPTEC, scale bar = 5 μm. ( l ) SEM image of OPTEC brush border, scale bar = 20 μm. ( m ) TEM image of brush border, scale bar = 1 μm.

Article Snippet: Figure 2 Optimizing OPTEC expansion. ( a ) Brightfield images showing OPTECs grown on plastic, matrigel, and laminin-511 substrates. ( b ) Heat map showing transporter expression of OPTECs between passages 0 (after isolation) and 5. ( c ) Brightfield images showing OPTEC populations at passages 0–5, scale bar = 20 μm. ( d ) Schematic view of the extracellular matrix (ECM), media, and media supplement conditions explored for optimizing OPTEC culture conditions. ( e ) Heat map comparing transporter expression as a function of the experimental conditions tested in ( d ).

Techniques: Staining, Marker, Membrane, Expressing

Journal: Scientific Reports

Article Title: 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptake

doi: 10.1038/s41598-022-19293-3

Figure Lengend Snippet: Improved transporter expression and polarization of 3D OPTECs-on-chip. ( a ) Heat map showing OCT2, OAT1, and OAT3 transporter expression for OPTECs and PTEC-TERT1s-on-chip (day 0, as seeded), after achieving confluency (day 7, perfusion), and one-week after achieving confluency on chip (day 14, perfusion). ( b ) General transporter analysis comparing OPTEC tubules normalized by PTEC-TERT1 tubules after day 14 of perfusion on chip, one sample t test, n = 6 tubules across 3 batches of OPTECs, *p < 0.05, **p < 0.01, ***p < 0.005. ( c , d) Immunofluorescence images showing OCT2 (green) and DAPI (blue) staining in OPTEC tubules after day 14 of perfusion on chip. ( e–f ) Immunofluorescence images of showing localization of OAT3 (green) and DAPI (blue) in OPTEC tubules after day 14 of perfusion on chip ( g , h ), Immunofluorescence images showing OCT2 (green) and DAPI (blue) staining in PTEC-TERT1 tubules after day 14 of perfusion on chip, and ( i , j ) Immunofluorescence images of showing localization of OAT3 (green) and DAPI (blue) in PTEC-TERT1 tubules after day 14 of perfusion on chip, scale bars = 20 μm.

Article Snippet: Figure 2 Optimizing OPTEC expansion. ( a ) Brightfield images showing OPTECs grown on plastic, matrigel, and laminin-511 substrates. ( b ) Heat map showing transporter expression of OPTECs between passages 0 (after isolation) and 5. ( c ) Brightfield images showing OPTEC populations at passages 0–5, scale bar = 20 μm. ( d ) Schematic view of the extracellular matrix (ECM), media, and media supplement conditions explored for optimizing OPTEC culture conditions. ( e ) Heat map comparing transporter expression as a function of the experimental conditions tested in ( d ).

Techniques: Expressing, Immunofluorescence, Staining

Journal: The Yale Journal of Biology and Medicine

Article Title: 2020 Evaluation of Portable Vision Screening Instruments

doi:

Figure Lengend Snippet: Comparison of seven portable vision screening instruments outlining the cost, amblyopia risk factor screened, and clinically useful characteristics.

Article Snippet: Optec 5500P differs from other instruments as its measurements of visual acuity and phoria require output from the child while the others objectively assess refraction, alignment, fixation, and media clarity based on a child’s ability to fixate on a target.

Techniques: Comparison, Battery, Transmission Assay